SOME INDICATORS FROM ANIMAL TISSUES

Some Aspects of the Desoxyribonuclease Activities of Animal Tissues

It has been found that many animal tissues contain "acid" desoxyribonucleases with pH optima near 5.2. A chemical method for the determination of this activity is described. The pancreatic desoxyribonuclease crystallized by Kunitz and shown to have a neutral pH optimum occurs in the pancreas together with the "acid" enzyme, but only the "neutral" enzyme occurs in the pancreatic juice. The ratio...

Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues.

Preparation of extracts from animal tissues.

The initial procedure in the isolation of an protein, a protein complex, or a subcellular organelle is the preparation of an extract that contains the required component in a soluble form. Indeed, when undertaking a proteomic study, the production of a suitable cellular extract is essential. Further isolation of subcellular fractions depends on the ability to rupture the animal tissues in such ...

RNA Extraction from Animal and Human\'s Cancerous Tissues: Does Tissue Matter?

The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

The distribution and properties of transhydrogenase from animal tissues.

where TPN+ and TPNH represent the oxidized and reduced forms oftriphosphopyridine nucleotide and DPN+ and DPNH represent the corresponding forms of diphosphopyridine nucleotide. Further studies by Kaplan, Colowick & Neufeld (1952, 1953) showed that the reactions were brought about by hydrogen, not electron, transfer and that the enzyme occurred in some animal tissues. The purpose of the present...